Search results for "Type IX-like collagen"

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Cloning and expression of a type IX-like collagen in tissues of the ascidian Ciona intestinalis

2002

Collagens are highly preserved proteins in invertebrates and vertebrates. To identify the collagens in urochordates, the total RNA extracted from the pharynx of the ascidian Ciona intestinalis was hybridized with a heterologous probe specific for the echinoderm Paracentrotus lividus fibrillar type I-like larval collagen. Using this probe, two main bands (i.e. 6 and 2.8 kb mRNA) were observed on Northern blot hybridization. The cDNA library prepared from poly(A)+RNA extracted from pharyngeal tissue was screened and a cDNA that specifies a type IX-like collagen was identified. This molecule presents a conceptual open reading frame for a protein containing 734 amino acids. In particular, we sh…

Transcription GeneticAscidianMolecular Sequence DataBiophysicsIn situ hybridizationcDNA libraryBiochemistryCollagen Type IXMiceStructural BiologyComplementary DNAGeneticsAnimalsHumansCiona intestinalisTissue DistributionNorthern blotAmino Acid SequenceRNA MessengerCloning MolecularType IX-like collagenPeptide sequencePhylogenyGene LibraryMessenger RNAbiologyBase SequenceSequence Homology Amino AcidcDNA libraryRNAbiology.organism_classificationMolecular biologyCiona intestinalismRNA localizationSequence Alignment
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